Measuring absolute expression with microarrays with a calibrated reference sample and an extended signal intensity range

摘要

Gene expression ratios derived from spotted-glass microarray exper- iments have become invaluable to researchers by providing sensitive and comprehensive indicators of the molecular underpinnings of cell behaviors and states. However, several drawbacks to this form of data have been noted, including the inability to relate ratios to absolute expression levels or to compare experimental conditions not measured with the same control. In this study we demonstrate a method for overcoming these obstacles. First, instead of cohybridiz- ing labeled experimental and control samples, we hybridize each sample against labeled oligos complementary to every microarray feature.