Restriction mapping in nanofluidic devices

摘要

We have performed restriction mapping of DMA molecules using restriction endonucleases in nanochannels with diameters of 100-200 nm. The location of the restriction reaction within the device is controlled by electrophoresis and diffusion of Mg~(2+) and EDTA. We have successfully used the restriction enzymes Smal, Sacl, and Pacl, and have been able to measure the positions of restriction sites with a precision of ≈1,5 kbp in 1 min using single DNA molecules.