首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Remote activation of biomolecules in deep tissues using near-infrared-to-UV upconversion nanotransducers
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Remote activation of biomolecules in deep tissues using near-infrared-to-UV upconversion nanotransducers

机译:使用近红外到紫外上转换纳米传感器远程激活深层组织中的生物分子

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摘要

Controlled activation or release of biomolecules is very crucial in various biological applications. Controlling the activity of biomolecules have been attempted by various means and controlling the activity by light has gained popularity in the past decade. The major hurdle in this process is that photoactivable compounds mostly respond to UV radiation and not to visible or near-infrared (MR) light. The use of UV irradiation is limited by its toxicity and very low tissue penetration power. In this study, we report the exploitation of the potential of NIR-to-UV upconversion nanoparticles (UCNs), which act as nanotransducers to absorb NIR light having high tissue penetration power and negligible phototoxicity and emit UV light locally, for photoactivation of caged compounds and, in particular, used for photo-controlled gene expression. Both activation and knockdown of GFP was performed in both solution and cells, and patterned activation of GFP was achieved successfully by using upconverted UV light produced by NIR-to-UV UCNs. In-depth photoactivation through tissue phantoms and in vivo activation of caged nucleic acids were also accomplished. The success of this methodology has defined a unique level in the field of photo-controlled activation and delivery of molecules.
机译:生物分子的受控活化或释放在各种生物学应用中非常关键。在过去的十年中,已经尝试了各种方法来控制生物分子的活性,并且通过光来控制活性已经得到普及。该过程中的主要障碍是光活化化合物主要对紫外线辐射做出反应,而不对可见光或近红外(MR)光做出反应。紫外线照射的使用受到其毒性和非常低的组织穿透力的限制。在这项研究中,我们报告了利用NIR到UV上转换纳米颗粒(UCNs)的潜力,该纳米颗粒可作为纳米传感器吸收具有高组织穿透力和可忽略的光毒性的NIR光,并局部发出UV光,从而用于笼蔽化合物的光活化特别是用于光控基因表达。在溶液和细胞中均进行了GFP的激活和敲除,并且通过使用由近红外到紫外UCN产生的上转换的紫外光成功实现了GFP的模式激活。还完成了通过组织体模的深度光激活和笼状核酸的体内激活。这种方法的成功定义了光控激活和分子传递领域的独特水平。

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