Pea formaldehyde-active class III alcohol dehydrogenase: Common derivation of the plant and animal forms but not of the corresponding ethanol-active forms (classes I and P)

摘要

A plant class III alcohol dehydrogenase (or glutathione-dependent formaldehyde dehydrogenase) has been characterized. The enzyme is a typical class III member with enzymatic parameters and substrate specificity closely related to those of already established animal forms. K_m values with the pea enzyme are 6.5 μM for NAD~+, 2 μM for S-hydroxymethylglutathione, and 840 μM for octanol versus 9, 4, and 1200 μM, respectively, with the human enzyme.