Production, specificity, and functionality of monoclonal antibodies to specific peptide-major histocompatibility complex class II complexes formed by processing of exogenous protein

摘要

Several unanswered questions in T cell im- munobiology relating to intracellular processing or in vivo antigen presentation could be approached if convenient, spe- cific, and sensitive reagents were available for detecting the peptide-major histocompatibility complex (MHC) class I or class II ligands recognized by αβ T cell receptors. For this reason, we have developed a method using homogeneously loaded peptide-MHC class II complexes to generate and select specific mAb reactive with these structures using hen egg lysozyme (HEL) and I-A~k as a model system. mAbs specific for either HEL-(46-61)-A~k or HEL-(116-129)-A~k have been isolated.