Three-dimensional diffuse x-ray scattering from crystals of Staphylococcal nuclease

摘要

We have developed methods for obtaining and characterizing three-dimensional maps of the reciprocal- space distribution of diffuse x-ray scattering from protein crystals, and have used the methods to study the nature of disorder in crystals of Staphylococcal nuclease. Experimen- tally obtained maps are 99.5/100 complete in the reciprocal- space resolution range of 10 A deg -2.5 A deg, show symmetry consis- tent with the P4_1 space group of the unit cell, and are highly reproducible.