Red and far-red fluorescent proteins for in vivo imaging

摘要

TagRFP and mKate2 are the brightest monomeric fluorescent proteins in the red part of the spectrum. Both reporters demonstrate high pH-stability, fast maturation at 37℃ and excellent performance in fusions. As an additional advance, mKate2 is extremely photostable under both widefield and confocal illumination, which makes it ideally suited for long-term time-lapse imaging. A variety of expression vectors encoding TagRFP and ml