How to build a fluorescent protein

摘要

To be useful for super-resolution microscopy, a fluorescent protein must have all the properties necessary for standard imaging: it can't be toxic; it must label the intended target; it must be biologically active at the same temperatures as mammalian cells (no small feat, given that these proteins generally come from sea creatures living in chilly waters); it must be bright; and its fluorescence must stand out from background.