Conformational control of DNA target cleavage by CRISPR-Cas9

摘要

Cas9 is an RNA-guided DNA endonuclease that targets foreign DNA for destruction as part of a bacterial adaptive immune system mediated by clustered regularly interspaced short palindromic repeats (CRISPR)(1,2). Together with single-guide RNAs3, Cas9 also functions as a powerful genome engineering tool in plants and animals(4-6), and efforts are underway to increase the efficiency and specificity of DNA targeting for potential therapeutic applicatione(7,8). Studies of off-target effects have show