Adsorption of tributyltin by tributyltin resistant marine Pseudoalteromonas sp. cells

摘要

The isolate, Pesudoalteromonas sp. TBT1, could grow to overcome the toxicity of tributyltin chloride (TBTC1) up to 30 μM in the absence of Cl~- in the medium until the cells reached an exponential phase of growth. The viability, however, was reduced after the cells reached a stationary phase. The degradation products, such as dibutyltin (DBT) and monobutyltin (MBT), were not detected in the growth medium, indicating that the isolate has no ability to degrade TBT into less toxic DBT and MBT. Up to about 10~(7.5) TBT molecules were adsorbed by a single cell. The observation of morphological changes with an electron microscope showed that the cell surface became wrinkled after exposure to the lethal concentration of 10 mM TBTC1. These results indicate that the resistance of the isolate toward the toxicity of TBTC1 is not related to the unique cell surface, which seems to play an important role in preventing the diffusion of TBTC1 into the cytoplasm.