Light Activated Recombination

摘要

Methods to remotely regulate gene expression have revolutionized the study of gene function by enabling one to selectively turn on or turn off genes in a temporally defined manner.However,many genes,such as morphogens or neuronal guidance cues,can only properly elicit their actions through their precise spatial and temporal patterning in tissues.Several groups have used various strategies to photocaged biopolymers(DNA,RNA,or proteins)as a way to control gene expression in a spatiotemporally defined manner.