Binary Malachite Green Aptamer for Fluorescent Detection of Nucleic Acids

摘要

The design of the probe allows the hybridization event to be accompanied by an increase in fluorescence, which is easily and instantly detectable. The binary probe reliably discriminates 41 out of 42 possible single nucleotide substitutions in 14-mer DNA analyte with extremely high discrimination factors ( > 20) for more than half of the possible substitutions. These high discrimination abilities of the biMGA probe is demonstrated at room temperature in a buffer simulating physiological conditions. Moreover, biMGA consists entirely of unmodified RNA and thus can be expressed in living cells as a gene product. All these facts taken together make biMGA a promising instrument for highly selective fluorescent monitoring of nucleic acids in cell culture and in vivo.