Simulated Unfolded-State Ensemble and the Experimental NMR Structures of Villin Headpiece Yield Similar Wide-Angle Solution X-ray Scattering Profiles

摘要

With the advent of powerful synchrotron sources,solution X-ray scattering is being increasingly used to get basic information about the structure of proteins in the native aqueous milieu.In addition to analysis of the long-range structure of polypeptides (such as their radius of gyration),solution scattering has also been used to study interdomain correlation and intradomain geometry,including the secondary structural content of some proteins.