One-Electron Oxidation of DNA Oligomers That Lack Guanine;Reaction and Strand Cleavage at Remote Thymines by Long-Distance Radical Cation Hopping

摘要

Damage to DNA is caused by oxidative processes that result in loss of an electron and the concomitant generation of a radical cation that migrates through the nucleobases of the duplex by a hopping mechanism.The radical cation is eventually trapped in a reaction with H_2O or O_2 that results in the conversion of a base to a mutated form.A defining characteristic of the one-electron oxidation of duplex DNA is reaction at G_n(n=1-3)sites that is detected as strand cleavage following chemical or enzymatic treatment.