Engineered Biosynthesis of Macrolide Derivatives Bearing the Non-Natural Deoxysugars 4-epi-D-Mycaminose and 3-N-Monomethylamino-3-Deoxy-D-Fucose

摘要

A growing appreciation of the essentiality of deoxysugars for the physiological functions of cell surface polysaccharides and the biological activities of many secondary metabolites has led to a surge of investigations of the biosynthesis of deoxysugars. These studies have facilitated the rational manipulation of the deoxysugar biosynthetic machinery to generate a diverse array of new glyco-conjugates with potential clinical applications. The success of these engineering strategies hinges on the identification and exploitation of the substrate flexibilities of pathway enzymes. Interestingly, the substrate flexibilities of several natural product glycosyltransferases have been demonstrated and subsequently exploited for the biosynthesis of a variety of natural product glycoforms.