Synthesis and Biophysical Characterization of Oligonucleotides Containing a 4'-Selenonucleotide

摘要

The replacement of the sugar ring (4'-) oxygen of nucleosides and nucleic acids by sulfur has been the subject of numerous studies. In the case of DNA and 2'-deoxy-2'-fluoroarabinonucleic acid (2'F-ANA), sulfur substitution causes a conformational switch to northern, RNA-like conformations that leads to interesting biological properties. 4'-S-DNA can be amplified by PCR and can direct transcription in mammalian cells.3 The conformational shift observed for 4'-S-FANA has made it useful for siRNA gene silencing. 4'-S-RNA has also been applied to modification of aptamers and siRNA.