Recyclable Nanobiocatalyst for Enantioselective Sulfoxidation: Facile Fabrication and High Performance of Chloroperoxidase-Coated Magnetic Nanoparticles with Iron Oxide Core and Polymer Shell

摘要

Magnetic nanoparticles (MNPs) have received increasing attention in biorelated research due to several distinct properties such as high surface area to volume ratio, special magnetic behavior, and high dispersibility in various solvents. An important application for MNPs is biocatalysis which has a profound impact on green and selective chemical syntheses. Enzyme-coated nanosized magnetic particles used as a nanobiocatalyst could be simply recycled under an external magnetic field. Compared with enzymes immobilized on micrometric supports, a nanobiocatalyst could achieve a much higher enzyme loading capacity and significantly enhanced mass transfer efficiency. An attractive approach for fabricating a nanobiocatalyst is to immobilize the enzyme on iron oxide MNPs which are cheap and biocompatible. Several enzymes such as hydrolase, glucose oxidase, and alcohol dehydrogenase have been thus far immobilized on such MNPs.