Atomic-Level Mapping of Antibody Epitopes on a GPCR

摘要

Identifying the structures that contribute to monoclonal antibody (mAb) binding sites (epitopes) within native G protein-coupled receptors (GPCRs) can be useful for developing topological models of the accessible receptor surface, for selecting the most relevant mAbs for therapeutic, diagnostic, and research applications and for distinguishing the intellectual property positions of otherwise similar mAbs. While conventional site-directed mutagenesis studies can identify individual amino acid residues that are critical to mAb binding, defining comprehensive epitopes is difficult and time-consuming for these structurally complex proteins.