2-aminopurine-modified Abasic-site-containing Duplex Dna For Highly Selective Detection Of Theophylline

摘要

Aptamers (short nucleic acid-binding species) have emerged as promising candidates for molecular recognition events on account of their significant ability to bind a large number of ligands. High affinities and specificities of aptamers toward target ligands are generally achieved by a combination of molecular-shape complementarity, hydrogen bonding, and stacking interactions. Such binding events typically involve ligand-induced structural changes in the aptamers, resulting in the formation of unique secondary structures responsible for the ligand binding, of which the internal-loop and stem-loop structures are representative of active sites for binding events.