Kinetic determination of morin nanoamounts

摘要

A kinetic-spectrophotometric method is proposed for the determination of morin. The method is based on the inhibition effect of morin on the oxidation of C_6H_5OONa by hydrogen peroxide in the presence of the complex Fe(Ⅱ)-AA(ascorbic acid), which acts as a catalyst. The concentration range for the determination of morin is one of the lowest achieved so far (a linear calibration graph was obtained for morin from 2.255-22.55 ng cm~(-3)). The limit of detection of the method is 0.28 ng cm~(-3). The relative error ranges between 1.42 to 5.10 % for the given concentration interval. Kinetic equations are proposed for the investigated process. The effects of certain foreign ions upon the reaction rate were determined in order to assess the selectivity of the method. The major advantages of this kinetic-spectrophotometric assay are its sensitivity, selectivity, reproducibility, speed and simplicity.