Effect Of Intravenous Infusion Of 0.9% Saline On Neutrophil Activation In Healthy Volunteers

摘要

Objective. Activated neutrophils have been implicated as effectors of tissue injury and end-organ damage in adult respiratory distress syndrome and multi-organ dysfunction syndrome. Recent research has shown that hypertonic saline may abrogate neutrophil activation and in turn attenuate end-organ damage, while lactated Ringer's solution causes neutrophil activation. There has been little research investigating the effects of iso-osmolar solutions such as 0.9% saline on neutrophil activation. The aim of this study was to determine the effect of an infusion of 0.9% saline on the activation of human neutrophils in healthy volunteers. Material and methods. A 2-1 bolus infusion of 0.9% saline was administered to eight healthy volunteers over a 1-h period after an overnight fast. Venous blood samples were taken on three occasions: pre-infusion (time 0), on completion of the infusion (time 1) and 1 h later (time 2). Neutrophil activation was quantified by flow cytometric analysis of CD lib expression. Submaximal and maximal stimulation of neutrophils were achieved using different concentrations of N-formyl-methionyl-leucyl-phenylalanine. Results. Median (interquartile range) baseline neutrophil activation (median fluorescence intensity of CD lib expression) at times 0, 1 and 2 was 178 (162-237), 180 (152-350) and 175 (129-212), respectively. Corresponding values for submaximal stimulation were 620 (412-921), 711 (342-972) and 586 (364-924), and those for maximal stimulation were 2410 (1913-2846), 2410 (1968-2945) and 2455 (1823-2645). The differences at each time point were not significant for any of the parameters. Conclusion. Infusion of 0.9% saline does not confer the potentially beneficial effects of the inhibition of neutrophil activation witnessed with hypertonic saline in other studies.