The Effect of As_2O_3 on the Epression of Drug-resistance Molecule in Malignant Neoplasmas

摘要

Objective: To detect the action of arsenic trioxide (As_2O_3) on the expression of Tumor drug-resistant molecule. Methods: APL cell line MR_2 resistant to all-trans retin-oic acid (ATRA ) was put into research, while APL cell line NB_4 was used for control. The im-munocytochemical assays were used to detect the expressions of P-glycoprotein (P_(gp)) and Gluta-thione S-transferase ( GST) . Results: Not only the expression of P_(gp) in MR_2 cell line (30%-40%) was significantly higher than that in NB4 cell line (10%-20% ) (P < 0.001), but also the expression of GST in MR_2 cell line (60. 4 +- 4.0 )-( 66.5 +- 4.4) was significantly higher than that in NB4 cell line (28.3 +- 5.6)-(31.2 +- 5. l)(P < 0.05). As_2O_3 at the concentration of 0.5-2.0 μmol/l could significantly decrease the expression of P_(gp) and GSTπ, but could do nothing about the expression of GSTα and GSTμ. Conclusion: The lower expression of P_(gp) and GSTπ might be the sensitive indications of frustrating drug-resistance, while GSTα and GSTμ might not be the case. ATRA might be the substrates of P_(gp) transmission and GSTπ catalysis .