Effect of Intravascular Irradiation on Intimal Smooth Muscle Cells Proliferation and Apoptosis in Balloon Injuried Arteries

摘要

Objective: To investigate the effect of intravascular in radiation on the arterial wall smooth muscle cells (SMCs) proliferation and apoptosis after iliac artery bollom injury in figs. Methods: Twenty-seven miniature figs were divided into three groups. All pigs underwent iliac artery balloon over-stretch. An~(192) Ir source through afterloader was positioned at the injuried segments to give 10 Gy in 9 pigs and 20 Gy in the other 9 pigs, and the rest 9 pigs were, used as control group. The pigs were killed on the 3rd, 10th and 28th days respectively for observation. The injured segments were processed to examine SMCs proliferation by proliferation cell nuclear antigen (PCNA) and apopto-sis by terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL). Results: PC-NA index analysis has some that SMCs proliferation in neointima was significantly inhibited in irradiation group on the 10th and 28th days. The value for intimal SMCs apoptosis in control vs 10 Gy and 20 Gy irradiation groups were: (1. 185+-0. 49)% vs (2. 27+-0. 49)%(P>0. 05) and (1. 85+-0. 49)% vs (2. 53+-0. 45)%(P<0. 05), at the 10th day; (1. 61+-0. 35)% vs (3. 11+-0. 51)%(P<0. 05), and (1.61+-0. 35)% vs (7. 05+-1. 82)% (P<0. 05), on the 28th day. In irradiated arteries, the maximal incidence of intimal SMCs apoptosis was (7. 05+--1. 82)% in 20 Gy group vs (3. 11+-0. 51)% in 10 Gy group (P<0. 05), on the 28th day. In the same dose irradiation group, the incidence of intimal SMCs apoptosis was higher on the 28th day than that on the 10th day. Conclusion: Intra-arterial gamma irradiation can inhibit intimal SMCs proliferation and stimulate SMCs apoptosis in balloon-in jured arteries. These may be contributive to prevention of restenosis of arteries after balloon injury.