Characterization of mesh size asymmetry in hydrogel membranes using confocal micoscopy

摘要

Hydrgel membranes with mesh size asymmetry, created by cross-linking poly(vinyl alcohol) (PVA) under a glutaraldehyde gradient, were characterized with laser scanning confocal fluorescence microscopy. A fluroescent label, 5-{[4,6-dichlorotriazin-2-yl] amino}-fluorescein (DTAF) was attached to PVA, which then was used to prepare homogeneous and asymmetric hydrogel membranes.