β-cyclodextrin functionalized poly (5-amidoisophthalicacid) grafted Fe_3O_4 magnetic nanoparticles: A novel biocompatible nanocomposite for targeted docetaxel delivery

摘要

Thiol-lactam initiated radical polymerization (TLIRP) was successfully employed to prepare poly-N- 5-acrylamidoisophthalicacid grafted onto Fe_3O_4 magnetic nanoparticles (MNPs@PAIP).β-Cyclodextrin (CD) was then conjugated to the carboxylic groups of the prepared MNPs via carbodiimide activation. Subsequently, tumor-targeting folic acid (FA) was attached to the hydroxyl groups of CD on the surface of the latter MNPs to increase the site-specific intracellular delivery. The prepared MNPs were fully characterized by FTIR, VSM, TGA, XRD, FE-SEM and TEM. Docetaxel (DTX) as hydrophobic anticancer drug was loaded via host-guest inclusion complexation with CD and the release profile of the system was studied at different pH. The effect of MNPs on the cell viability was evaluated for the human embryonic kidney normal cell line (HEK293) as well as HeLa and MDA-MB-231 cancerous cell lines and the results did not show any apparent cytotoxic effect In comparison, DTX loaded MNPs reduced the growth of HeLa and MDA-MB-231 cells more than free DTX. Intracellular uptake ability of DTX loaded MNPs was also studied using fluorescent microscopy and showed cellular uptake about 90% after 4 h treatment.