The Effects of Magnesium Sulfate on Fetal Rats of FGR and the Expression of Caspase-3 in the Placenta of Maternal Rat

摘要

To investigate the effect of magnesium sulfate on the fetal rats of FGR and the expression of caspase-3 in the placenta of maternal rat; To explore the mechanism of using magnesium sulfate to cure the FGR. Establish model of FGR by a way of passive smoking: giving the maternal rats different agent of magnesium sulfate by subcutaneous injection; low agent group (300 mg/kg) , high agent group (600 mg/kg). Concentration of magnesium sulfate was monitored. The expression of caspase-3 was measured by RT-PCR and immunohistochemistry technology. Both of the concentrations of magnesium sulfate in high and low agents group are higher than the FGR group (P < 0. 01); the weight of the placenta and fetal rat in high agent group are higher than the FGR group (P < 0. 05 and P < 0. 01); the expression of mRNA and protein of caspase-3 in the two agent group is higher than the FGR group (P < 0. 05 respectively); concentration of magnesium sulfate in the maternal rat blood correlate to the weight of fetal rat (r=0. 899, P = 0. 038) and the expression of caspase-3 in the placenta of maternal rat (r=-0.747, P = 0.033; r= - 0.915, P= 0.001). The research suggests that the weight of fetal rat could be increased by treatment of magnesium sulfate. Because it would imfrmove the placental function by depressing the expression of caspase-3.