Microorganism selection and biosurfactant production in a continuously and periodically operated bioslurry reactor

摘要

A continuous-flow reactor(CSTR) and a soil slurry-sequencing batch reactor (SS-SBR) were maintained in 81 vessels for 180 days to treat a soil contaminated with diesel fuel (DF). Con- centrations of Candida tropicalis, Brevibacterium casei, Flavobacterium aquatile, Pseudomonas aeruginosa, and Pseudomonas fluorescens were determined using fatty acid methyl ester (FAME) analysis. DF removal (biological and volatile) and biosurfactant concentrations were measured. The SS-SBR encouraged the growth of biosurfactant-producing species relative to the CSTR. Counts of biosurfactant-producing species (C. tropicallis, P. aeruginosa, P, fluoresecens) relative to total microbial counts were 88% in the SS-SBR and 23/100 in the CSTR.