Somatic embryogenesis and plantlet regeneration in callue cultures derived from mature zygotic embryos of slash pine

摘要

White, translucent, and mucilaginous embryogenic callus was initiated in cultured mature zygotic embryo explants of two different seed sources of slash pine (Pinus elliottii) on several culture media containing auxin and cytokinin. Somatic proenbryos were induced on media containing 2, 4-D and BA. Maturation was successfully achieved on medium supplemented with ABA. Somatic embryos germinated into regeneration plantlets on DCR medium containing activated charcoal.