Comparative Evaluation of Culture- and BAX Polymerase Chain Reaction-Based Detection Methods for Listeria spp. And Listeria Monocytogenes in Environmental and Raw Fish Samples

摘要

Two commercial polymerase chain reaction (PCR)-based Listeria detection systems, the BAX for Screening/Listeria monocytogenes and the BAX for Screening/Genus Listeria, and a culture-based detection system, the Biosynth L. Monocyto- genes Detection System (LMDS), were evaluated for their ability to detect L. Monocytogenes and Listeria spp. In raw ingre- dients and the processing environment. For detection of L. Monocytogenes from raw fish, enrichment was performed in Listeria enrichment broth (LEB), followed by polating on both Oxford agar and LMDS L.