EFFECT OF HYDROGEN PEROXIDE PRETREATMENT ON THE ENZYMATIC HYDROLYSIS OF CELLULOSE

摘要

In this work, the effect of oxidation pretreatment on the enzymaticnhydrolysis of cellulose in tobacco stems was evaluated. A comparison wasnmade between the effect of H2O2 pretreatment and conventional alkali pretreatmentnon the weight loss of cellulose, lignins and hemicelluloses of tobacconstems. The effects of H2O2 concentration, pretreatment time and temperaturenon the enzymatic hydrolysis of tobacco stems were investigated. This pretreatmentncould give a higher recovery of cellulose and better removal of lignin andnhemicellulose than alkali-only pretreatment. Compared with the untreatednstems, the yield of reducing sugar pretreated by H2O2 pretreatment (pH 11.5,n0.6% H2O2) for 9 h at 60C increased 347.2%. The results of scanning electronnmicroscope and X-ray diffraction analysis also showed that the structural andncrystalline properties of the tobacco stems were changed by pretreatment, andnthey were in favor of the following enzymatic hydrolysis. The optimal pretreatmentnconditions were 60C of temperature, 0.6% of H2O2 concentration and 9 hnof time, which were calculated by orthogonal experiments.