Authentication of Bungarus parvus and Its Adulterants by DNA Molecular Method Using Diagnostic Primer

摘要

In order to develop a more applicable DNA molecular marker identification method for the authentication of Dungarees pervious and its adulterants, DNA templates were extracted from Dungarees pares, adulterants of the crude drugs and their original animals. Cyt b gene fragment was amplified from all these templates respectively using universal primers. PCR predictors were purified and sequenced directly. After sequence alignment and comparing the sequence of Dungarees pervious to its adulterants, we find that Cyt b gene is a good molecule marker for authentication of the crude drugs since interspecific differentiation is more evident than that of interspecies in DNA sequence of the gene.