Biohydrogen production from paper industry wastes by SSF: A study of the influence of temperature/enzyme loading

摘要

The present work focused in assessing the hydrogen production from pretreated wastes of the paper industry (PIW) by simultaneous saccharification and fermentation (SSF) using anaerobic biofilms developed in natural fibers (ixtle) at different conditions. Anaerobic sludge from brewery wastewater treatment was used for biofilms and they were developed in plastic spheres covered with fiber cord from ixtle. The solid wastes of paper industry were previously pretreated with H2SO4 at 2.5% (v/v) at 120 degrees C by 30 min. The solids pretreated were hydrolyzed and fermented in batch reactors. All reactors were kept at an initial pH of 5.0 and three levels of enzyme loadings (10, 40 and 70 FPU/mL) and temperatures (35, 45, 55 degrees C) were assessed. The maximum hydrogen obtained (60.75 mmo/h*g volatile solids) was at 45 degrees C and 70 FPU/mL, moreover, no methane was detected in all cases. (C) 2019 Hydrogen Energy Publications LLC. Published by Elsevier Ltd. All rights reserved.