Surface Plasmon Resonance-based Dna Biosensor For Arsenic Trioxide Detection

摘要

Surface plasmon resonance-based biosensor has been fabricated using self-assembled monolayer (SAM) of β-merceptoethanol (MCE) deposited on gold (Au) substrate. The double stranded calf thymus deoxyribonucleic acid (dsCT-DNA) has been immobilised onto MCE/Au electrode using N-ethyl-N'-(3-dimethylaminopropyl) carbodiimide and N-hydroxysuccinimide (EDC/NHS) chemistry. This dsCT-DNA/MCE/Au bioelectrode has been characterised using cyclic voltammetry, scanning electron microscopy (SEM) and X-ray photoelec-tron spectrometry (XPS), respectively. The detection limit of arsenic trioxide has been estimated as 0.01 × 10~(-6) g mL~(-1).