Role of IFN-γ and IL-6 Cytokines and Their Association in Determining Susceptibility to Chronic Idiopathic Urticaria

摘要

Chronic Idiopathic Urticaria (CIU) is a common skin disorder, which may occur spontaneously. The aim of the present study was to assess the serum levels of interferon (IFN)-y and interleukin (IL)-6 and to examine the association of IFN-γ + 874 T/A and IL-6-174 G/C cytokine gene polymorphisms. To accomplish this, ELISA-based cytokine serum levels of IFN-γ (n = 30) and IL-6 (n = 30) in CIU patients (n = 100) and Healthy Controls (HC) (n = 200) were performed. Amplification refractory mutation system-polymerase chain reaction (ARMS-PCR) was performed to verify the positional significance. A significant (p＜ 0.0001) increase in the serum cytokine levels of IFN-γ and IL-6 was recorded in CIU patients compared to HC. The AT and TT genotypes of IFN-γ and GG genotype of IL-6 were found to be significantly associated with CIU. In conclusion, our findings show a significant increase in the cytokine levels of IFN-γ and IL-6, highlighting their regulatory role in the development of disease. In addition to this, association studies have revealed that TT genotype of IFN-γ + 874 T/A and GG genotype of IL-6-174 G/C were susceptible towards the CIU.