Development of a novel method for isolating Cryptosporidium parvum from lean and fat beef and detecting carcass surfaces

摘要

This paper describes the first reported method for the recovery and detection of Cryptosporidium parvum oocysts from beef carcasses. C. parvum oocysts were mobilized from beef surfaces into phosphate buffer saline-Tween 80, and subsequently recovered from this suspending medium by membrane filtration. Oocysts were removed from the membrane, concentrated by centrifugation, labelling with an FITC conjugated monoclonal antibody and enumerated using fluorescence microscopy. The study compared 'pulsification', a novel method, with 'stomaching' an established method, as processes for resuspending the target microorganisms from beef. Both yielded similar levels of recovery of oocysts, but pulsification produced less suspended sample debris, allowing easier oocyst detection.