Deutscher Lebensmittelchemikertag 2014 in Giessen

摘要

Bioactive substances, such as endocrine disrupting chemicals (EDCs), are consumed unknowingly through the food, food supplements, cosmetics or commodities. They can bind to human estrogen receptor α/β (ERα/β) and thereby activate or inhibit the endocrine system of humans or wildlife. Coupling of bioassays with TLC/HPTLC has already been shown, but results were not satisfactory due to the diffuse zones formed. The bioassay workflow was substantially improved. Within 5 h, 20 samples can be analyzed in parallel with regard to any estrogen-active compounds. Sharp-bounded, narrow compound zones linking to the biological effect were obtained. This advancement was demonstrated for quantitation of food for EDCs reacting with the human estrogen receptor and detectable down to the femtogram-per-zone range in the chro-matogram (ng/kg range in samples).