Application of acetylacetone chelation solid-phase extraction to GFAAS measurements of trace amounts of beryllium in livers and muscles of poultry and livestock

摘要

Trace amounts (0.004-0.55 ng) of beryllium (Be) in a dried bovine liver sample (20 mg) can be accurately determined by graphite-furnace atomic absorption spectrophotometry (GFAAS) after treating with microwave digestion (HNO_3/H_2O_2) at 85 ℃ for 10 min and using acetylacetone as a chelating agent in the presence of an acetate buffer (pH 6.0). The method detection limit (MDL, 3a) for Be was found to be 0.18 ng g~(-1) and the limit of quantification (LOQ, 10σ) was found to be 0.60 ng g~(-1); the calibration graph was linear up to 27 ngg~(-1). The Be contents measured in four liver and four muscle samples (BCR CRM-185R bovine liver, BCR CRM-384 pork muscle, and six samples collected in Kaohsiung, Taiwan, ROC) were between 2.3 and 4.7 ng g~(-1). Good spiked recoveries (96.0-103.0%) were obtained for these eight samples with a relative standard deviation (RSD, n = 3) ≤3.0%. The method could be applied to measurements of Be in livers and muscles of poultry and livestock.