A novel chemiluminescence sensor for determination of quercetin based on molecularly imprinted polymeric microspheres

摘要

A novel flow injection chemiluminescence (Fl-CL) sensor for determination of quercetin using the system of luminol-NaOH-H_2O_2 based on molecularly imprinted polymeric microspheres (MlPm) as recognition element is established. The quercetin MlPm was synthesised by precipitation polymerisation using ace-tone as solvent, acrylamide (AM) as functional monomer, ethylene glycol dimethacrylate (EGDMA) as cross-linker and 2,2-azobisisobutyronitrile (AIBN) as initiator. And the polymers' properties were charac-terised. The FI-CL optimised experimental were obtained and the possible mechanism was discussed. The CL intensity responded linearly to the concentration of quercetin ranged from 1.4 × 10~(-6) to 1.6 × 10~(-4) mol/L with a detection limit of 9.3 × 10~(-7) mol/L (3σ). The relative standard deviation (RSD) for determination was ranged from 2.72% to 3.31%. On the basis of speediness and sensitivity, the sensor is reusable and has a great improvement in selectivity. As a result, the new sensor had been successfully applied to the determination of quercetin in drug samples.