Simultaneous multiresidue determination of mycotoxins in cereal samples by polyvinylidene fluoride membrane based dot immunoassay

摘要

The development of polyvinylidene fluoride (PVDF) membrane-based dot immunoassay for rapid and simultaneous detection of multi-mycotoxins in cereal samples is described. To facilitate the simultaneous identification of multiple mycotoxins in a single test, membrane reaction zones were constructed using PVDF membrane, rubber fences and scotch tape. The cut-off level for this method, assessed visually, were 20,60,1000,20, and 250 μg kg~(-1) for Aflatoxin B_1 zearalenone, deoxynivalenol, ochratoxin A, and fumon-isins B_1, respectively, and the final results can be obtained within 10 min. This simultaneous method was designed to be simple, with no time-consuming cleanup procedure, and good accuracy and reproducibil-ity were obtained in recovery experiments. These results suggest that this method could be a useful on-site screening tool for the rapid detection of multiple mycotoxins in cereal samples without special instrumentation.