Determination of anti-oxidative histidine dipeptides in poultry by microchip capillary electrophoresis with contactless conductivity detection

摘要

A home-made microchip electrophoresis (MCE) device was used to quantitate two biologically important histidine dipeptides, carnosine and anserine, using capacitively coupled contactless conductivity detection ((CD)-D-4), at pH 2.7. The (CD)-D-4 detector exhibited a linear response to both carnosine and anserine in the range of 0-200 mu M for the individual dipeptides and in the range of 0-100 mu M for each dipeptide when both were present as a mixture. The limit of detections (LOD) for the dipeptides in the mixture were 0.10 mu M for carnosine and 0.16 mu M for anserine. Standard addition was used to detemine the accuracy of the method. For carnosine and anserine the recoveries were in the range of 96.7 +/- 4.9-106.0 +/- 7.5% and 95.3 +/- 4.5-105.0 +/- 5.1% in thigh muscle and 97.5 +/- 5.1-105.0 +/- 7.5% and 95.3 +/- 5.4-97.3 +/- 5.6% in breast muscle, respectively. (C) 2016 Elsevier Ltd. All rights reserved.