Authentication of swordfish (Xiphias gladius) by RT–PCR and FINS methodologies

摘要

In the present study, two methods for the authentication of swordfish (Xiphias gladius) were developed. The first one is based on a TaqMan probe real-time PCR technology using the cytochrome oxidase subunit I (COI), while the second one is based on the phylogenetic analysis of DNA sequences (forensically informative nucleotide sequencing, FINS) using the cytochrome b (cyt b) gene fragment. Both techniques can be applied depending on the laboratory equipment and allow the detection of fraudulent or unintentional mislabelling of this species. The developed methodologies were validated and subsequently were applied to 30 commercial samples labelled as swordfish or X. gladius in order to determinate whether the species used for their manufacturing corresponded to this species. These tools are useful to clarify questions related to the correct labelling of commercial products and to verify the correct traceability in commercial trade and for fisheries control.