THE EFFECT OF STRESS ON TOXICANT-DEPENDENT CYTOCHROME P450 ENZYME RESPONSES IN THE ARCTIC CHARR (SALVELINUS ALPINUS)

摘要

In the present study we investigated the effect of stress and cortisol on cytochrome P450 (CYP) expression in Arctic chart exposed to benzo[a]pyrene (BaP). Expression of hepatic CYP1A and CYP3A was monitored 8 d after a single oral dose of BaP (10 mg/kg fish) and compared to that in unexposed fish. During this period the fish were subjected to one of the following stress regimes: no stress, no stress and cortisol implantation, 10 min of daily handling and confinement stress, and confinement stress during the last 6 h before sampling. In BaP-exposed fish daily stress resulted in significantly lower (53/100) CYP1A protein levels as compared to those in unstressed fish.