The Effect of Nickel(II) Ions on the Growth and Bioaccumulation Properties of Escherichia coli

摘要

The influence of nickel(II) ions concentration on the growth and nickel(II) bioaccumulation properties of Escherichia coli (cultivated in the LB medium) was studied. When the investigated initial concentration of nickel(II) was in the range of 20-300 mg L~(-1), it was found that the growth of E. coli was delayed obviously with the increasing nickel(II) ion concentration, while the accumulation capacity increased until the maximum was obtained. FIIR spectrum showed that the peak near 3300 cm~(-1) shifted remarkably, and simultaneously, the peaks at 1660 cm~(-1) strengthened according to the nickel(II) concentration. As shown by the X-ray photoelectron spec-troscopy analysis, the nitrogen, sulphur, and phosphorus contents of the cultivated cell were reduced, while the content of oxygen was increased. The biologic characteristics of the microorganism did not change as shown in the transmission electron microscope images.