Characterization of the mouse islet-specific glucose-6-phosphatase catalytic subunit-related protein gene promoter by in situ footprinting correlation with fusion gene expression in the islet-derived βTC-3 and hamster insulinoma tumor cell lines

摘要

Glucose-6-phosphatase(G6Pase)is a multicomponent system located in the endoplasmic reticulum comprising a catalytic subunit and transporters for glucose-6-phos- phate, inorganic phosphate, and glucose. We have re- cently clones a novel gene that encodes an islet-specific G6Pase catalytic subunit-related protein(GIRP)(Ebert Et al., Diabetes 48:543-551, 1999). To begin to investi- Gate the molecular basis for the islet-specific expression Of the IGRP gene, a series of truncated IGRP-chloram- Phenicol acetyltransferase(CAT)fusion genes were Transiently transfected into the islet-derived mouse ΒTC-3 and hamster insulinoma tumor cell lines.