Effect of miR-384-targeting LINC00491 on proliferation, migration and invasion of tongue squamous cell carcinoma cells

摘要

Purpose: To investigate the effect of long-chain non-coding RNA LINC00491 (LncRNA LINC00491) on the proliferation, migration and invasion of tongue squamous cell carcinoma (TSCC) cells, and the underlying mechanism. Methods: Real-time quantitative polymerase chain reaction (qRT-PCR) was applied to determine the ex pressions of LINC00491 and micro-RNA-384 (miR-384). Furthermore, LINC00491 and miR-384 were transfected into CAL-27 cells, while cell cycle was analyzed using flow cytometry. Cell proliferation was determined by methyl thiazolyl diphenyl-tetrazolium (MTT) assay. Cell migration and invasion were evaluated using Transwell experiments. The relationship between LINC00491 and miR-384 was confirmed using double luciferase reporting assay, while protein ex pression levels of P21, Ki67, E-cadherin, N-cadherin, and vimentin were assayed with Western blotting. Results: The ex pression of LINC00491 increased in TSCC tissues (p 0.05). The proportion of cells in G1-phase increased, while the proportion of cells in S-phase decreased (p 0.05). There was decrease in cell survival, cell migration and cell invasion (p 0.05). The protein ex pression levels of Ki67, N-cadherin, and vimentin were lowered, while those of P21, E-cadherin protein were increased (p 0.05). Transfection of LINC00491 and miR- 384 reduced the proportion of cells in G1 phase, but increased the proportion of cells in S-phase (p 0.05). Moreover, cell survival, migration and invasion were increased. The protein ex pressions of Ki67, N-cadherin, and vimentin rose, while those of P21 and E-cadherin decreased (p 0.05). Conclusion: LINC00491 promotes the proliferation, migration and invasion of TSCC cells by inhibiting miR-384. This finding provides a potential target for the treatment of TSCC.