Proteomics study on the protective mechanism of soybean isoflavone against inflammation injury of bovine mammary epithelial cells induced by Streptococcus agalactiae

摘要

This study aimed to verify the anti-inflammatory effect of soybean isoflavones (SI) on the inflammatory response induced by Streptococcus agalactiae ( S. agalactiae ) of bovine mammary epithelial cells (bMECs) and to elucidate its possible mechanism. BMECs were pretreated with SI of different concentrations (20, 40, 60, 80, 100?μg/mL) for 0.5, 3, 6, 9, 12, 15, 18, 24?h. And then, S. agalactiae was used to infect bMECs for 6?h (MOI?=?50:1) to establish the inflammation model. Cell viability, growth curves of S. agalactiae , cytotoxicity, and S. agalactiae invasion rate were determined. A proteomics technique was used to further detect differential proteins and enrichment pathways. SI (40?μg/mL) improved the viability of bMECs at 12?h ( p ?