Activated pathogenic Th17 lymphocytes induce hypertension following high-fructose intake in Dahl salt-sensitive but not Dahl salt-resistant rats

摘要

Objectives: High-salt or high-fructose intakes are risk factors for hypertension via oxidative stress and inflammation. T helper (Th)17 lymphocytes play an important role in the development of hypertension. We tested the hypothesis that activation of pathogenic Th17 lymphocytes induces hypertension after high-fructose intake in Dahl salt-sensitive (SS) but not Dahl salt-resistant (SR) rats.Methods: Eight-week-old male SS and SR rats were offered 20% fructose solution or tap water only for 4 weeks. Systolic blood pressure was measured by the tail-cuff method. T lymphocytes (Th17 and T regulatory (Treg)) profiling was determined via flow cytometry. The expression of Th17 -related (interleukin (IL)-17A, IL-17RA,IL-23R and retinoic acid receptor-related orphan receptor (ROR) γt) and Treg-related (IL-10, CD25, forkhead box (Fox)P3, and TGF-?) factors were measured via ELISA or qRT-PCR. Th17 lymphocytes isolated from high fructose-fed SS rats were intraperitoneally injected into recipient SS and SR rats. Moreover, recombinant IL-23 protein was subcutaneously injected into SS and SR rats to induce hypertension.Results: High-fructose intake induced hypertension via the activation of pathogenic Th17 lymphocytes in SS but not SR rats. Injection of activated Th17 lymphocytes isolated from fructose-fed SS rats induced hypertension via increase of serum IL-17A in only recipient SS rat. In addition, injection of IL-23 induced hypertension via activation of pathogenic Th17 lymphocytes in only SS rats.Conclusion: Activation of pathogenic Th17 lymphocytes induces hypertension after high-fructose intake in SS but not SR rats. These results implicate that immunologic tolerance plays an important role in the protection against hypertension in SR.? 2020. Published by The Company of Biologists Ltd.