Illumina short-read sequencing data, de novo assembly and annotations of the Drosophila nasuta nasuta genome

摘要

TheDrosophila nasuta nasuta(D. n. nasuta) is a member ofnasutasubgroup ofimmigransspecies group ofDrosophilawidely distributed across South-East Asia and central to Southern Africa. It displays morphological similarities with other members of thenasutasubgroup with which it has a recent divergence history. The genomic DNA ofD. n. nasutaCoorg strain was paired-end sequenced using Illumina HiSeq 2500 technology to obtain a draft genome assembly of 145.64 Mb. The generated assembly retrieved 93.6% of the conserved dipteran BUSCO orthologs. Approximately 85% of theab initiopredicted proteins exhibit sequence similarity to the proteins ofD. albomicanswhich is the closest annotated species. This draft genome sequence is a valuable resource toDrosophilageneticists and evolutionary biologists to understand molecular organisation of the genome and its evolution during early stages of speciation.