This study aimed to analyze prognostic significance of aldehyde dehydragenase 1 (ALDH1) and Nodal expression in patients with colorectal cancer. ALDH1 and Nodal expressions were observed based on the immunohistochemistry staining from 108 colorectal cancer patients. Scores were given to the staining intensity and percentage of positive cells, and sum of two scores for each case was used to define the groups of ALDH1 and Nodal. We also investigated the protein and mRNA levels of ALDH1 and Nodal by Western blot and qRT-PCR assays. The results were analyzed with the clinicopathologic parameters of these patients. The results indicated that expressions of ALDH1 and Nodal were significantly correlated with the differentiation degree, metastasis, number of tumor positive lymph nodes and AJCC stage. ALDH1 was inclined to express more in the worse differentiated degrees, lymph node metastasis, and worse AJCC stage of colorectal cancer patients. And the expression of Nodal was inversely compared with ALDH1.While the expression of ALDH1 was inversely correlated with the Nodal (r?=??0.709, P??0.01).
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机译：本研究旨在分析醛脱氢酶1（ALDH1）和结直肠癌患者节点表达的预后意义。基于来自108名结肠直肠癌患者的免疫组织化学染色来观察ALDH1和节点表达。给予染色强度和阳性细胞百分比的分数，并且每种情况的两种评分的总和用于定义Aldh1和Nodal的基团。我们还通过Western印迹和QRT-PCR测定研究了AldH1和Nodal的蛋白质和mRNA水平。通过这些患者的临床病理学参数分析结果。结果表明，Aldh1和Nodal的表达与分化度，转移，肿瘤阳性淋巴结数和AJCC阶段显着相关。 Aldh1倾向于更差异的分化度，淋巴结转移以及较差的结肠直肠癌患者的阶段。与Aldh1相比，Nodal的表达与Aldh1相比。当AlDH1的表达与节点（ = = ?? 0.709， p ？<？0.01）。
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