Cytotoxicity and Mineralization Potential of Four Calcium Silicate-Based Cements on Human Gingiva-Derived Stem Cells

摘要

The aim of this study was to evaluate the cytotoxicity and mineralization potential of four calcium silicate-based cements on human gingiva-derived stem cells (GDSCs). The materials evaluated in the present study were ProRoot MTA (Dentsply Tulsa Dental Specialties), Biodentine (Septodont), Endocem Zr (Maruchi), and RetroMTA (BioMTA). Experimental disks of 6 mm in diameter and 3 mm in height were produced and placed in a 100% humidified atmosphere for 48 h to set. We evaluated the cytotoxic effects of the cements using methyl-thiazoldiphenyl-tetrazolium (MTT) and live/dead staining assays. We used a scratch wound healing assay to evaluate cell migratory ability. Mineralization potential was determined with an Alizarin red S (ARS) staining assay. In the MTT assay, no significant differences were found among the ProRoot MTA, Biodentine, and control groups during the test period (p 0.05). The Endocem Zr and RetroMTA groups showed relatively lower cell viability than the control group at day 7 (p 0.05). The RetroMTA group had slower cell migration compared to the control group at days 3 and 4 (p 0.05). In the ARS assay, the ProRoot MTA, Biodentine, and RetroMTA groups exhibited a significant increase in the formation of mineralized nodules compared to the Endocem Zr and control groups on day 21 (p 0.05). In conclusion, the four calcium silicate-based cements evaluated in the present study exhibited good biological properties on GDSCs. ProRoot MTA, Biodentine, and RetroMTA showed higher mineralization potential than the Endocem Zr and control groups.